Challenges in Biological Cryo Electron Microscopy: Faraday Discussion 240 (Faraday Discussions #240) (Hardcover)

Challenges in Biological Cryo Electron Microscopy: Faraday Discussion 240 (Faraday Discussions #240) By Royal Society of Chemistry (Other) Cover Image

Challenges in Biological Cryo Electron Microscopy: Faraday Discussion 240 (Faraday Discussions #240) (Hardcover)


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This is book number 240 in the Faraday Discussions series.

Developments in cryo-electron microscopy are creating new opportunities within structural biology and there is currently great interest in developing cryo-EM as a core tool for atomic level structural biology. Many structural techniques can give atomic or near atomic level information, but lack the ability to study proteins within a near-native environment, for example within a cellular compartment. Cryo-EM provides this opportunity, but despite the recent massive improvements in single particle cryo-EM, obtaining sub-2 structural information is still a major challenge. Cryo-electron microscopy has undergone significant developments in microscope design, camera technology and data processing regimes, but there are significant challenges that remain and opportunities to explore, many of which must be tackled by the community as a whole, rather than by individual groups. For example, sample preparation is central to electron microscopy and is currently a significant bottleneck in many experiments, and there are significant problems with ensuring the integrity of the field in terms of dealing with inherently low signal-to-noise images.

This volume brings together leading researchers from the UK and the international cryo-electron microscopy community to discuss current developments and new challenges in the field.

In this volume the topics covered include:

  • Sample preparation in single particle cryo-EM
  • Pushing the limits in single particle cryo-EM
  • Tomographic analysis, CLEM
  • Map/model validation and machine learning in EM
Faraday Discussions documents a long-established series of Faraday Discussion meetings which provide a unique international forum for the exchange of views and newly acquired results in developing areas of physical chemistry, biophysical chemistry and chemical physics. The papers presented are published in the Faraday Discussion volume together with a record of the discussion contributions made at the meeting. Faraday Discussions therefore provide an important record of current international knowledge and views in the field concerned. The latest (2020) impact factor of Faraday Discussions is 4.008.
Product Details ISBN: 9781839164255
ISBN-10: 1839164255
Publisher: Royal Society of Chemistry
Publication Date: December 12th, 2022
Pages: 338
Language: English
Series: Faraday Discussions